Multiplicity of DNA end resection machineries in chromosome break repair.

DNA end resection machineries for human DNA break repair MRN constitute two − RPA − BLM − MRN and EXO 1 − RPA − DNA 2 − BLM

DNA end resection machineries for human DNA break repair MRN constitute two − RPA − BLM − MRN and EXO 1 − RPA −

DNA End Resection: Nucleases Team Up with the Right Partners to Initiate Homologous Recombination.

The repair of DNA double-strand breaks by homologous recombination commences by nucleolytic degradation of the 5'-terminated strand of the DNA break. This leads to the formation of 3'-tailed DNA, which serves as a substrate for the strand exchange protein Rad51. The nucleoprotein filament then invades homologous DNA to drive template-directed repair. In this review, I discuss mainly the mechani...

Relationship of DNA degradation by Saccharomyces cerevisiae exonuclease 1 and its stimulation by RPA and Mre11-Rad50-Xrs2 to DNA end resection.

Homologous recombination is a major pathway for repair of DNA double-strand breaks. This repair process is initiated by resection of the 5′-terminated strand at the break site. In yeast, resection is carried out by three nucleolytic complexes: Mre11-Rad50-Xrs2, which functions at the initial step and also stimulates the two processive pathways, Sgs1-Dna2 and Exonuclease 1 (Exo1). Here we invest...

53BP1 regulates DSB repair using Rif1 to control 5' end resection.

The choice between double-strand break (DSB) repair by either homology-directed repair (HDR) or nonhomologous end joining (NHEJ) is tightly regulated. Defects in this regulation can induce genome instability and cancer. 53BP1 is critical for the control of DSB repair, promoting NHEJ, and inhibiting the 5' end resection needed for HDR. Using dysfunctional telomeres and genome-wide DSBs, we ident...